Separation and enrichment of geniposidic acid from leaves of Eucommia ulmoides by aqueous two-phase flotation / 中草药

ABSTRACT

Objective: Ethanol/NaH2PO4 aqueous two-phase gas solvent sublation coupled with response surface methodology was developed for the separation/enrichment and analysis of geniposidic acid from the leaves of Eucommia ulmoides. Methods: For the aqueous two-phase flotation project, the flotation effects of different solvent, salts, flotation rate, flotation time, amount of crude extract and so on were deeply investigated; Furthermore, a Box-Behnken central composite test design was studied to determine the best flotation process conditions of GPA, and three factors including the mass fraction of NaH2PO4, the mass fraction of ethanol, and the flotation rate were selected based on the single factor test in the design. A 100 times scale-up experiment also was tested. Results: The optimal conditions of the flotation were as follows the mass fraction of NaH2PO4 was 25%, the mass fraction of ethanol was 20%, the amount of crude extract was 5 g, the flotation rate was 30 mL/min, and the flotation time was 20 min. Under the optimal condition, the flotation efficiency was 97.88%, and the enrichment factor was 27.34; The 100 times scale-up results showed that the flotation efficiency of GPA was 95.60%, and the RSD value was 0.77%. Conclusion: The method with ethanol/NaH2PO4 aqueous two-phase gas solvent sublation is suitable for the separation and enrichment of the active ingredients from E. ulmoides because of its high distribution coefficient and large enrichment factor.