Interaction of Shunaoxin Dripping Pill and Warfarin sodium on anticoagulant applications / 中草药

ABSTRACT

Objective: To study the anticoagulant effect of Shunaoxin Dropping Pill (SDP) alone or in combination with Warfarin sodium on platelet aggregation and provide the experimental support for the clinical safety of medication. Methods: Experiments were carried out in control group, low-dose SDP group, high-dose SDP group (45.36 and 90.72 mg/kg), and Warfarin sodium (clinical equivalent dose 0.4 mg/kg) group, Combined administration with low-dose SDP and high-dose SDP groups+clinical equivalent dose of Warfarin sodium. We determined the platelet aggregation rate of rats in the control group, low-dose SDP group, and high-dose SDP group in vitro by turbidimetry. We made iac administration of SDP and Warfarin sodium for 3 d, and took abdominal aortic blood for separating the platelets and used nephelometry for determining platelet aggregation rate; We separated serum and used semi-automatic coagulation analyzer to test APTT, PT, and TT. Results: Compared with the control group, SDP had the strong anti-platelet aggregation in vitro and could inhibit the increase of platelet aggregation in vivo slightly with the dose increasing. SDP could prolong APTT, PT, and PT; Compared with the single drug group, the combination therapy had no obvious effect on the platelet aggregation but could significantly extend the APTT, PT, and TT. Conclusion: SDP has some inhibition on platelet aggregation with anticoagulant effect; Combined with Warfarin sodium, SDP has a synergistic interaction on anticoagulant effect with Warfarin sodium.