Quality comparison on Farfarae Flos from various origins by UPLC based on multi-components content determination method / 中草药

ABSTRACT

Objective: To establish a UPLC method for simultaneously determining 12 components and evaluating the quality of Farfarae Flos from various origins. Methods: The UPLC method was achieved by BEH C18 column (100 mm × 2.1 mm, 1.7 μm) using a mobile phase made up of acetonitrile (A)-0.03% trifluoroacetic acid in water (B). The flow rate and detection wavelength were 0.5 mL/min and 254 nm, respectively.The column temperature was 40 ℃. Coefficients of variation (CV) were calculated, then PCA and HCA were applied to analyzing Farfarae Flos from different origins. Results: The method for content determination was in agreement with methodological requirements. The results showed that CV values of different components differed greatly, and the hyperoside showed the largest CV value at 0.91. PCA and HCA analysis revealed the wild and cultivated Farfarae Flos were different, and the wild samples contained more chlorogenic acid, caffeic acid, rutin, hyperoside, 3,4-dicaffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic, and tussilagone. Conclusion: There exist the significant differences among Farfarae Flos from various origins, and their CV values are also different. In addition, the wild samples also differ from those of the cultivated ones. To guarantee the safety and effectiveness in clinical, the impact of chemical differences of different Farfarae Flos on the clinical efficacy should be further studied.