Identification of cultured and natural Astragali Radix based on fingerprint of monosaccharides / 中草药

ABSTRACT

Objective: To establish monosaccharide fingerprint of carbohydrates in Astragali Radix, and find the specificities of saccharides between natural and cultured Astragali Radix to provide the basis for evaluating the quality of Astragali Radix in different cultivation patterns. Methods: Monosaccharide fingerprint from 24 kinds of different cultivations of Astragali Radix based on carbohydrates hydrolysis followed by chromatographic analysis was founded. The data of monosaccharide fingerprint were statistically analyzed based on principal component analysis (PCA) and cluster analysis (CA). Results: The carbohydrates content of natural Astragali Radix was apparently higher than the cultured, one and the natural and cultured Astragali Radix could be distinguished by molar ratios of mannose, glucose, and arabinose (the molar ratio of mannose and arabinose was more than 3.51 and the ratio of glucose and arabinose was more than 41 in cultured Astragali Radix, while the ratio of mannose and arabinose was less than 3.51 and the ratio of glucose and arabinose was less than 41 in natural Astragali Radix). Conclusion: The results not only supply the foundation for screening the quality indicators for Astragali Radix, but also provide the new ways of quality evaluation for medicinal materials in which carbohydrates are major bioactive components.