HPLC fingerprint on roots of Polygonum multiflorum from different habitats / 中草药

ABSTRACT

Objective: To establish an HPLC-fingerprint method for evaluating the quality of the root of Polygonum multiflorum from different habitats in China. Methods: A separation was performed on an Agilent extend C18 chromatographic column (250 mm × 4.6 mm, 5 μm) with gradient elution. The mobile phase consisted of acetonitrile-formic acid water solution (0.1%). And the mobile phase was at flow rate of 1.0 mL/min. The detection wavelength was at 280 nm. HPLC fingerprint chromatograms of 16 batches of crude material of the roots of P. multiflorum were analyzed. Chemometric methods including principle component analysis (PCA), and clustering analysis (CA) were used through the software of Matlab for common peaks. And the content of 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside was determined by HPLC. Results: The similarities of the 16 batches of samples were between 0.936 and 0.998. This showed that the quality of the roots of P. multiflorum from different habitats in China was stable. Eighteen common peaks were identified by HPLC/Q-TOF/MS. The results of FCA and CA were consistent with similarity evaluation. The content of 2,3,5,4'-tetrahydroxy stilbene-2-O-β-D-glucoside was consistent with principle component loading factors. Conclusion: The method is simple reliable, and it can provide a standard and guidance for the quality control of the roots of P. multiflorum.