Flavonoids from flower buds of Rosa rugosa / 中草药

ABSTRACT

Objective: To identificate the bioactive natural products, and study the chemical constituents in the flower buds of Rosa rugosa. Methods: The chemical constituents in the flower buds of R. rugosa were isolated by silica gel, MCI-gel resin, Sephadex LH-20 column chromatography, and high performance liquid chromatography (HPLC) methods. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. Results: Nine flavonoids were isolated from the flower buds of R. rugosa, and identified as 8-acetyl-4',7-dimethoxy-6-methyl-flavone (1), kaempferol (2), kaempferol-3-O-β-D-glucopyranoside (3), kaempferol-3-O-β-D-rutinside (4), quercetin (5), quercetin-3-O-β-D-glucopyranoside (6), rutin (7), luteolin (8), and luteolin-7-O-β-D-glucopyranoside (9). Conclusion: Compound 1 is a new compound and it displays the cytotoxicity against NB4, SH-SY5Y, PC3, A549, and MCF7 cells with IC50 values of 6.8, 5.6, 2.2, 1.8, and 7.4 μmol/L, respectively. Compound 9 is isolated from R. rugosa for the first time.