Analysis on endogenous metabolites in cecal tissue of CUMS-induced depression rats after Xiaoyao San intervention by GC-MS metabolomics / 中草药

ABSTRACT

Objective: To investigate the changes of endogenous metabolites in cecal tissue of CUMS-induced depression rat model interfered by Xiaoyao San via GC-MS metabolomics. Methods: Chronic unpredictable mild stress (CUMS) rat model was used and Xiaoyao San and fluoxetine hydrochloride (fluoxetine) treatment were intervention drug. The endogenous metabolites were analyzed and identified by NIST 05 database or authentic standards. The final data were introduced into SIMCA-P 13.0 software package for multivariate analysis. Results: The CUMS rat model was successfully duplicated, the metabolites of alanine, serine, and glutamic acid were reduced at fluoxetine group and Xiaoyao San group, and palmitic acid and stearic acid were increased in fluoxetine group in cecal tissue of rats after drug intervention, compared with model group with significant difference (P < 0.01 or 0.05). Conclusion: The endogenous metabolites were changed by Xiaoyao San intervention significantly in cecal tissue of CUMS rats, which provides a basis for further study of the mechanism of Xiaoyao San treatment of depression.