Effects and action mechanism of Fuzheng Xiaoliu Decoction on cerebral glioma orthotopic transplantation model in rats / 中草药

ABSTRACT

Objective: To observe the inhibitory effect of Fuzheng Xiaoliu Decoction (FXD) on cerebral glioma. Methods: Rat glioma orthotopic transplantation model was established by inoculating C6 glioma cells to brain right caudate nucleus in situ of Wistar rats, and the rats in treatment group were ig administered with FXD for consecutive 14 d. After the last administration, blood was taken to measure whole blood viscosity and plasma viscosity, serum immunoglobulin (IgG, IgA, and IgM), tumor necrosis factor-α (TNF-α), interleukin-2 (IL-2), and interferon-γ (IFN-γ) levels; All rats were sacrificed after last administration to collect whole blood; The brain was taken and weighed, brain index was calculated, tumor length and short diameters were measured, and tumor volume and inhibitory rate were calculated; The tumor tissue was taken, vascular endothelial growth factor (VEGF) and prostaglandin E2 (PGE2), insulin-like growth factor-1 (IGF-1) levels were determined. Results: All three doses (1.2, 2.4, and 3.6 g/kg) of FXD could inhibit rat glioma tumor volume. It could reduce the contents of IGF-1, PGE2, VEGF in tumor tissue and serum IgG, elevated the level of serum IgA, IgM, TNF-α, IL-2, and IFN-γ to varying degrees. FXD (3.6 g/kg) can reduce whole blood viscosity at low shear (P < 0.05, 0.01). Conclusion: FXD has significant antitumor effect on C6 glioma, and the mechanism of antitumor effect may be the inhibition of tumor angiogenesis and tumor cell proliferation, enhancing immune response, and reducing blood viscosity.