Effect of fermentation time on antibacterial activity of Mirabilitum Praeparatum and screening of its antibacterial active parts / 中草药

ABSTRACT

Objective: To investigate the effect of fermentation time on antibacterial activity of Mirabilitum Praeparatum (MP) and screen the antibacterial active parts from MP with the optimal fermentation time. Methods: Using test tube in vitro antibacterial test and flat germicidal test, the pathogenic bacteria activities of MP with different fermentation time (7, 15, 22, 30, 50 d) were studied, and the optimal fermentation time was screened. The methanol extracts from MP with fermentation time of 15 d and its aqueous and 95% ethanol elution parts were prepared. The antibacterial and bactericidal effects of methanol extracts from MP and its aqueous and 95% ethanol elution parts were compared. Results: The 15 d fermentation MP had the strongest antibacterial activity. The 95% ethanol elution parts from MP had the strongest antibacterial activity on nine kinds of common pathogens, especially for the oral and upper respiratory tract pathogens. Conclusion: Fermentation time has obvious effect on antibacterial activity of MP, and 95% ethanol elution parts of macroporous adsorption resin from MP may be active parts.