Quantitative determination of phenyl propanoides from barks of Syringa dilatata grown in DPR of Korea / 中草药

ABSTRACT

Objective: To isolate phenyl propanoides from the barks of Syringa dilate, identify their structures, and determine the contents by reliable HPLC. Methods: Phenyl propanoides were extracted with methanol, and isolated by silica gel chromatography. The structures were elucidated by spectroscopic analysis including NMR, MS, UV, and IR. Shimpack CLC C18 (150 mm × 6 mm, 5 μm) column chromatography was employed with gradient elute of 1% acetic acid and methanol, and the column temperature was set at 30℃. The flow rate was 1.0 mL/min and the detection wavelength was set at 267 nm. Results: Two phenyl propanoides were isolated from the barks of S. dilata grown in DPR of Korea and identified as syringin (1) and coniferin (2). The linear range of syringin was 0.2-6.0 mg/mL, the correlation co-efficient was 0.999 8, and the average recoveries were 99.8%, 100.6%, and 100.9%. The results showed that the content in the barks was 8 folds of inner parts. There was no difference in locations and the content of syringin increased in winter, while decreased in summer. Conclusion: The elucidation of phenyl propanoides existence in S. dilata could be a fundamental for the search of new herbal materials. The method is simple, rapid, and reliable. It can be used to determine the content of syringin in herbal medicines.