Callus induction and determination of total alkaloid content from Fritillaria ebeiensis var. purpurea / 中草药

ABSTRACT

Objective: To study the combination and proportion for the explants in NaClO and the suitable hormone induced by callus of Fritillaria ebeiensis var. purpurea. The total alkaloids content variations of raw herb and callus were investigated. Methods: The fresh bulbs of F. ebeiensis var. purpurea were used as the exprimental materials and NaClO and antibiotics as disinfectants. It was cultured in vitro in MS medium with plant hormones 2,4-D + 6-BA and 2,4-D + KT by orthogonal test design. Alkaloids contents in original bulb and callus were measured with UV. Results: The best way to sterilization was at a concentration of 5% NaClO with 8 min. The optimal hormone levels was 2,4-D 0.5 mg/L + 6-BA 2.0 mg/L. The alkaloid contents of raw herbs and callus were 1.46% and 1.09%. Conclusion: Callus induced by tissue culture technique are soft, luster, and transparent, which are suitable for subculture. Total alkaloids content in callus is less than that in raw herb.