Research on SRAP molecular markers in germplasm resources of Coix lacryma-jobi / 中草药

ABSTRACT

Objective: To study the 25 germplasm resources of Coix lacryma-jobi by the technique of SRAP molecular markers. Methods: Taking genome DNA in the leaves of C. lacryma-jobi extracted by modified CTAB method as template. Six ideal primer pairs were selected from 88 primer pairs by using the optimized SRAP-PCR reaction system and the amplification of 25 germplasm resources by SRAP-PCR were carried out. Results: The total 66 clear and repeatable polymorphic bands were obtained, and the polymorphic rate was up to 93%. The coefficient of genetic similarity among each species was calculated by using NTSYPC 2.1 software. The results indicated that the coefficient of genetic similarity of these materials ranged from 0.48 to 0.82. Conclusion: The 25 populations of C. lacryma-jobi could be clustered into four groups.