Identification of chemical constituents in Ophiocordyceps xuefengensis sp. nov. by HPLC-Q-TOF-MS/MS / 中草药

ABSTRACT

Using the HPLC-Q-TOF-MS/MS to analyze and identify the main components in the ethanol extract of Ophiocordyceps xuefengensis sp. nov. The ethanol extract of O. xuefengensis sp. nov. was preparaed by using the ultrasonic methods, the main components in the extracts were separated by using the gradient elution method with RP-HPLC, Yuexu AQ-C18 column (250 mm × 4.6 mm, 5 μm); The positive and negative electro spray ionization (ESI) source was used for determine the chromatographic effluent, the main chromatographic peaks are assigned by Q-TOF. Based on the standards of MS/MS and compared with the reference results, 28 compounds, containing mannitol, adenosine, ergosterol, sitosterol, amino acid, fatty acid, and sugar, were identified. HPLC-ESI-TOF-MS/MS method can qualitatively analyze the main components in O. xuefengensis sp. nov. from the retention time, UV spectrum, precious relative molecular weight, formula, secondary fragment ions, and so on. It is an effective and fast analysis method for determining the active compounds in O. xuefengensis sp. nov.