Study on preparation of silver nanoparticles by biomass reduction of traditional Chinese medicinal materials and their antibacterial activity / 中草药

ABSTRACT

Objective: To use the extracts from traditional Chinese medicinal materials (TCMM) for reduction of Ag+ to obtain the corresponding silver nanomaterials and study its antibacterial activity. Methods: Nano-silver solution was prepared by Rhei Radix et Rhizoma and other 15 kinds of TCMM extracts, the size and distribution of silver nanoparticles were studied using HPPS, TEM, and UV. The antibacterial activities of silver nanoparticles were investigated by disk agar diffusion method and double dilution method. Results: Preparation rate and reaction rate of silver nanoparticles prepared with the water extract of Rhei Radix et Rhizoma, Polygoni Multiflori Radix Praeparata, Moslae Herba were ideal. Prepared silver nanoparticles with diameter about 100 nm, good uniformity, and good dispersibility have the obvious inhibitory effect on the growth of Staphylococcus aureus and Escherichia coli. Among them, Polygoni Multiflori Radix Praeparata-silver nano has the strongest bacteriostatic effect. Conclusion: TCMM as a natural bio-reducing agents, can be used to prepare the silver nanoparticles.