Simultaneous determination of five compoents in Xiaokeping Tablets by HPLC / 中草药

ABSTRACT

Objective: By optimizing the chromatographic conditions, to establish a method of multi-target content analysis, the components of danshensu, protocatechuic aldehyde, salvianolic acid B, puerarin, and berberine hydrochloride in Xiaokeping Tablets were simultaneously determined. Methods: Agilent Zorbax Eclipse XDB-C18 (150 mm × 4.6 mm, 5 μm) chromatographic separation column was used, mobile phase was composed of acetonitrile (A), methanol (B), and 0.5% H3PO4 aqueous solution (C) in a linear gradient elution mode (0 min A 4%, C 96%; 7 min A 4%, B 1%, C 95%; 20 min A 10%, B 3%, C 87%; 30 min A 15%, B 3%, C 82%; 45 min A 25%, B 10%, C 65%; and 60 min A 30%, B 10%, C 60%). The flow rate was 1.0 mL/min, the column temperature was set at 30 ℃, and injection volume was 10 μL. The detection wavelength was set at 280 nm for danshensu, protocatechuic aldehyde, and salvianolic acid B, 250 nm for puerarin, and 265 nm for berberine hydrochloride. Results: There was a good linear relationship over a rang of 33.02-528.41 μg/mL for danshensu, 1.44-22.46 μg/mL for protocatechuic aldehyde, 374.51-5992.20 μg/mL for salvianolic acid B, 84.86-1357.82 μg/mL for puerarin, and 312.50-5000.00 μg/mL for berberine hydrochloride, respectively. The average recovery rates were 98.24% (RSD = 0.26%), 98.37% (RSD = 1.01%), 98.86% (RSD = 0.50%), 98.93% (RSD = 0.68%), 99.04% (RSD = 0.29%), respectively. Conclusion: The separation effect is good, the operation is simple and convenient, so as to achieve the better quality control of the products by this method.