Inhibitory effects of curcumin on hyphal development and biofilm formation of five kinds of non-Candida albicans / 中草药

ABSTRACT

Objective: To observe the inhibitory effects of curcumin on hyphal development and biofilm formation of five kinds of non-Candida albicans. Methods: Serial 2-fold dilution assay was used to determine the MICs of curcumin to C. tropicalis, C. glabrata, C. parapsilokis, C. krusei, C. guilliermondii; XTT assay was used to determine the SMIC50 of curcumin to the five non-C. albicans. Inverted microscope and scanning electron microscope (SEM) were applied to inspectting the morphological change of non-C. albicans treated by curcumin, The dilution method was applied to inspecting the hyphae around the colonies. Results: The MICs of curcumin to C. tropicalis, C. glabrata, C. parapsilokis, C. Krusei, C. guilliermondii were 64, 128, 256, 256, and 128 μg/mL, and the SMIC50 were 512, 512, >512, >512, and 512 μg/mL, respectively. We found that curcumin could inhibit the hyphal and biofilm formation of the five kinds of non-C. albicans by observation with inverted microscope and SEM, and curcumin could inhibit the hyphal development around the colonies by observation with optical microscope. Conclusion: Curcumin could inhibit the hyphal development and biofilm formation of the five kinds of non-C. albicans.