Protective effects of angelica polysaccharide on hippocampal neuron of rats with cerebral ischemia-reperfusion injury / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 558-561, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-854233
ABSTRACT
Objective:
To investigate the protection of angelica polysaccharide (APS) on hippocampal neuron in rats with cerebral ischemia-reperfusion (I/R) injury.Methods:
The model of cerebral I/R injury was established by suture method in rats. A total of 50 rats were randomly divided into five groups Sham-operation, cerebral I/R injury, high-dose APS (200 mg/kg), mid-dose APS (100 mg/kg), and low-dose APS (50 mg/kg) groups. APS was ig administrated 3 d before operation. At 24 h after reperfusion, learning and memory function was detected by step down test, the apoptosis of hippocampal neuron was observed by terminal deoxylnucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), and the expression of cleaved caspase-3, bcl-2, and bax in the hippocampus of rats was measured by enzyme-linked immunosorbent assay (ELISA).Results:
Compared with those in the Sham-operation group, the learning and memory function was notably impaired in the I/R injury group, the number of errors increased. The apoptosis of hippocampal neuron increased and the expression of cleaved caspase-3, bcl-2, and bax in the hippocampus remarkably increased in the I/R injury group. The APS could significantly improve the learning and memory function of rats with the cerebral I/R injury and remarkably delay the decrease of the number of errors and the decrease of the apoptosis rate in the hippocampus of rats with the cerebral I/R injury. And the APS could also cause a significant down-regulation of cleaved caspase-3 and bax expression, while up-regulation of bcl-2 expression in hippocampus of rats with the cerebral I/R injury.Conclusion:
APS has a neuroprotection on rats with the cerebral I/R injury. The neuroprotective mechanism of APS may involve in the inhibition of the neuronal apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2015
Type:
Article
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