Study on UPLC fingerprint of Alpiniae Oxyphyllae Fructus / 中草药

ABSTRACT

To establish the fingerprint of Alpiniae Oxyphyllae Fructus by UPLC. The chromatographic fingerprint was obtained with ACQUITY UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm) and gradient eluted with acetonitrile and aqueous formic acid. The flow rate was 0.5 mL/min, the sample room temperature and the column temperature were maintained at 10℃ and 30℃, respectively. The detection wavelength was set at 254 nm. The injection volume was 2 μ L. The common mode of UPLC fingerprint of Alpiniae Oxyphyllae Fructus was set up firstly. There were 20 common peaks in the fingerprints. Protocatechuic acid, α-hydroxymethyl furfural, oxyphyllenodiol A, teuhetenone A, teuhetenone A, chrysin, kaempferide, tectochrysin, and nootkatone were identified by comparing the retention time and their ultraviolet spectra. The similarities of 15 Alpiniae Oxyphyllae Fructus among 19 batches of Alpiniae Oxyphyllae Fructus were above 0.970. The method is rapid and efficiency. It can be used for the quality evaluation of Alpiniae Oxyphyllae Fructus.