Establishment of fingerprint for Xinjiang chrysanthemum and analysis on its components by UPLC / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1223-1226, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-854293
ABSTRACT
Objective:
In order to identify the authenticity and control the quality of Xinjiang chrysanthemum, the fingerprinting of it was established using UPLC and discussed in this paper.Methods:
Thirteen batches of Xinjiang chrysanthemum from different origins were analyzed by UPLC with catechin, luteoloside, quercetin, and 3, 5-di-O-caffeoyl quinic acid as reference substances. The chromatographic condition was performed on Waters ACQUITY UPLC HSS T3 C18 (150 mm × 2.1 mm, 1.8 μm) and eluted with mobile phase containing acetonitrile-pH 3.0 phosphoric acid solution. The flow rate was 0.2 mL/min with detection wavelength 280 nm in the temperature of 30℃.Results:
There were 17 peaks in the UPLC fingerprint of Xinjiang chrysanthemum and three components were identified by three peaks determined using reference substance.Conclusion:
The similarity of 13 batches is little difference, but is more than 0.90. The results showed that the UPLC fingerprint chromatogram could be used as a valuable method for the quality evaluation of Xinjiang chrysanthemum.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2015
Type:
Article
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