Preparation and in vitro evaluation of antibacterial peptides from Plutella xylostella poly(lactic-co-glycolic acid) nanoparticles / 中草药

ABSTRACT

Objective: To prepare the antibacterial peptides from Plutella xylostella-loaded nanoparticles based on poly(lactic-co- glycolic acid) (CA-PLGA-NPs) and evaluate its physicochemical property and in vitro release. Methods: CA-PLGA-NPs were prepared by S/W/O/W double emulsion method combined with high-pressure homogenization. The morphology, particle size, polydispersion index (PDI), drug loading, encapsulation efficiency (EE), and in vitro release of the nanoparticles were studied. Results: CA-PLGA-NPs were spherical or similarly spherical, and the average particle size, PDI, drug loading, and EE were (358.76 ± 22.51) nm, 0.168 1 ± 0.012 2, (10.50 ± 0.28)%, and (60.92 ± 1.58)%, respectively. And burst phenomenon was not significant. The drug delivery stable phase was 2-10 d. Conclusion: S/W/O/W double emulsion method combined with high pressure homogenization method is suitable for the preparation of CA-PLGA-NPs, and provides a pharmaceutical basis for CA administration.