Effects of three kinds of cyclodextrin on in vitro corneal penetration of curcumin / 中草药

ABSTRACT

Objective: To study the effects of β-cyclodextrin (β-CD), hydroxypropyl-β-CD (HP-β-CD), and sulfobutyl-ether-β-CD (SBE-β-CD) on in vitro corneal penetration of curcumin. Methods: Improved Franz-cells were used for the corneal diffusion test and the cumulative permeation quantity of curcumin in the corneal was determined by HPLC. Results: β-CD at 0.02%, 0.04%, 0.06%, and 0.08% improved the apparent permeability coefficient as much as 1.06, 1.03, 1.00, and 0.95 times. HP-β-CD at 0.02%, 0.04%, 0.06%, and 0.08% improved the apparent permeability coefficient as much as 1.16, 1.19, 1.17, and 1.12 times; HP-β-CD at 0.04% and 0.06% there was a significant deference compared with the control group (P < 0.05). SBE-β-CD at 0.02%, 0.04%, 0.06%, and 0.08% improved apparently the permeability coefficient as much as 1.06, 1.23, 1.08, and 0.90 times. SBE-β-CD at 0.04% there was a significant deference compared with the control group (P < 0.05). SBE-β-CD at 0.06% as penetration enhancer, release curve accorded with zero order release equation (r = 0.9978). The average of the corneal hydration is 81.7%. Conclusion: HP-β-CD at 0.04% and 0.06% could improve the apparent permeability coefficient as much as 1.19 and 1.17 times (P < 0.05). SBE-β-CD at 0.04% could improve the apparent permeability coefficient as much as 1.23 times (P < 0.05). SBE-β-CD as corneal penetration enhancers can release safely and effectively. As corneal penetration enhancers, those three kinds of cyclodextrin are without irritation to corneal.