Effect of vincristine on proliferation inhibiting and apoptosis inducing on BGC cells and study on their mechanisms / 中草药

ABSTRACT

The effect and possible mechanism of vincristine on human gastric cancer cell BGC were investigated in in vitro and in vivo experiments. MTS and Hoechst assay was used to evaluate the effects of vincristine on human gastric cancer cell proliferation and apoptosis, respectively. The proliferation of BGC cells was examined by cell proliferation assay; The apoptosis of BGC cells was assayed by Hoechst and flow cytometry; Cell cycle analysis was performed by flow cytometry. The expression level of cell proliferation, cell cycle, and apoptosis related proteins was determined by Western blotting. The treatment effect of vincristine was analyzed in nude mice. MTS and Hoechst assay showed vincristine could inhibit the growth of BGCs and promote the apoptosis of BGCs both in time-dependent and dose-dependent manners. The apoptosis rate of BGCs cells increased gradually and the cell cycle was arrested in G1 phase after treated with vincristine. Vincristine could downregulate the expression of phosphorylated-FAK, FAK, E2F1, CylinE2, CyclinD2, CDK2, CDK6, and activate apoptosis-related protein Caspase-3. Vincristine could suppress the growth of human gastric cancer in nude mice. Vincristine could inhibit the proliferation and induce the G1-arrest of BGC cells through its downregulation of cell proliferation and cell cycle related proteins and it also promotes the apoptosis of BGC cells through the activation of Caspase-3.