Cloning and expression analysis of flavonols synthetase gene from Carthamus tinctorius / 中草药

ABSTRACT

Objective: To clone the flavonol synthase (FLS) gene from Carthamus tinctorius (safflower) pigments and study its expression in different blossom periods. Methods: Primers were designed according to FLS which was selected from transcriptome sequencing results of safflower petal. Taking total RNA of safflower petal as template, FLS genes were amplified by RT-PCR and connected to PEASY-T1 carrier, and positive cloning was detected by PCR and then sequenced. Results: Sequencing results showed that 224 bp sequence was acquired, to which the Blast comparison was carried out on NCBI. The gene had the higher homology compared with the FLS from other species. Conclusion: The fragment of FLS gene is cloned from safflower, and PCR primers of safflower are designed based on FLS gene for real-time PCR in different blossom of periods different varieties in safflower. The results show that the expression of safflower FLS genes in the full bloom of auspicious red oil sisters line is the highest.