Simultaneous determination of chlorogenic acid, caffeic acid, and 6-O-p-coumaroyl-D-glucopyranose in Lygodii Herba by HPLC / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 273-275, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-854352
ABSTRACT
Objective:
To establish a method for the determination of chlorogenic acid, caffeic acid, and 6-O-p-coumaroyl-D-glucopyranose in Lygodii Herba.Methods:
The determination was developed on Phenomenex C18 column (250 mm × 4.6 mm, 5 μm), methanol-acetonitrile-1% glacial acetic acid (5590) was used as mobile phase, the detective wavelength was 325 nm, and the column temperature was 35 ℃.Results:
The linear ranges of chlorogenic acid, caffeic acid, and 6-O-p-coumaroyl-D-glucopyranose were 0.368-2.760, 0.064-0.480, and 0.102-0.765 μg, respectively. The average recoveries were 101.48% (RSD = 1.44%), 99.56% (RSD = 0.92%), and 101.73% (RSD = 1.62%), respectively.Conclusion:
The method is simple, accurate, and highly reproducible, which could be used for the quality control of Lygodii Herba.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2015
Type:
Article
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