Preparation and evaluaiton of bufalin coated PBCA nanoparticles / 中草药

ABSTRACT

Objective: To prepare poly-n-butylcyanoacrylate (PBCA) nanoparticles entrapped a Chinese medicine monomer of bufalin. Methods: Alcohol polymerization method was adopted to prepare bufalin PBCA nanoparticles and the preparation process was optimized by single factor experiment and orthogonal test design, while size distribution, morphology, encapsulation efficiency (EE), drug loading and hemolytic of the prepared nanoparticles were systemly analyzed. Results: The obtained nanoparticles were regular in shape with average particle size of (151.2 ± 6.1) nm, as well as a poly-dispersity index of 0.133 ± 0.024. The Zeta potential and EE of bufalin PBCA nanoparticles were (-7.09 ± 0.58) mV and (70.812 ± 6.139)%, respectively. Drug loading of PBCA nanoparticles is (1.990 ± 0.291)%. Moreover, less hemolysis and improved safety were appeared in the formulation group, comparing with the bulk drug group. Conclusion: Alcohol polymerization method, a method to prepare PBCA nanoparticles which exhibites good physical and chemical properties, can effectively reduce the exposure time of the Chinese medicine monomer in the acidic media. It may provide a new idea for the preparation of nanocarrier of easily hydrolyzed monomer.