Simultaneous determination of seven effective constituents from Shuxiong prescription by RP-HPLC / 中草药

ABSTRACT

Objective: To establish a RP-HPLC method for the determination of ligustrazine hydrochloride, ferulic acid, hydrosafflower yellow A, ginsenoside Rg1, ginsenoside Rb1, ginsenoside Re, and notoginsenoside R1 in Shuxiong prescription. Methods: The Inertsil ODS-SP (250 mm × 4.6 mm, 5 μm) was used. The mobile phase, consisting of acetonitrile-0.05% phosphoric acid solution, was programmed for a gradient elution. The flow rate was 1.0 mL/min, the detection wavelength was 203 nm, and the column temperature was 30℃. Results: The excellent linearity with correlation coefficients (r) of 0.999 3-0.999 9 was obtained. The average recoveries of the seven compounds were 97.25%-103.52% and all RSD values were less than 3%. Conclusion: The method appears to be simple, accurate, and well reproducible, which could be used for the simultaneous determination of the above-mentioned seven compounds in Shuxiong prescription.