Comparison of crude and prepared Polygonum multiflorum-induced idiosyncratic hepatotoxicity based on lipopolysaccharide model / 中草药

ABSTRACT

Objective: To investigate the effects of crude and prepared Polygonum multiflorum (PM) to liver injury based on idiosyncratic hepatotoxicity model co-treated with lipopolysaccharide (LPS) at a non-hepatotoxic dose, aiming to compare the difference between them. Methods: The Sprague-Dawley (SD) rats were used by ig administration, respectively with 50% alcohol extracts from crude or prepared PM alone or co-treated noninjurious dose of bacterial lipopolysaccharide (LPS, 2.8 mg/kg) via tail vein injection. Blood was collected from the inferior vena cava into a sodium citrate-anticoagulant tube, and the liver was removed. The plasma samples separated from the collected blood were used for analysis on ALT and AST activities and the isolated livers were used for histopathological assessment. Results: Both crude and prepared PM were nontoxic on normal SD rats under the dose of 17.28 g/kg, when co-treated with LPS, the liver injury was found at the dose of 1.08 g/kg of crude PM, however, 8.64 g/kg of prepared PM. Conclusion: When processed, the maximum safe therapeutic dosage of PM would be enlarged from 1.08 g/kg of crude PM that is close to clinical dosage to 8.64 g/kg of prepared PM, which is four times larger than the crude one in LPS model. It shows that toxicity of PM could be reduced by processing.