ISSR analysis on genetic diversities and phylogenetic relationships of Gynostemma pentaphyllun germplasm reseources / 中草药

ABSTRACT

Objective: To analyze the genetic diversities and phylogenetic relationships of Gynostemma pentaphyllun germplasm. Methods: Genetic diversities and phylogenetic relationships of 48 G. pentaphyllun germplasm from 12 provinces in different ecological environments were analyzed by inter simple sequence repeat (ISSR) molecular makers. Results: (1) Fifteen primers were selected from 100 primers which had clear and stable polymorphic bands. A total of 214 loci were obtained, including 206 loci, on average, amplification site of each primer 14.27 and polymorphic loci (PPL) was 96.26%. The averagely observed number of alleles (Na), effective number of alleles (Ne), Shannon information index (I) and Nei's genetic index (H) were 1.9626, 1.3358, 0.2211, and 0.3598, respectively. The enetic similarity coefficient (GS) ranged from 0.57 to 0.96. Cluster annlysis with UPGMA method showed that the 48 testing materials were divided into four groups at the level GS 0.72. Conclusion: The results of ISSR analysis reveal that G. pentaphyllun germplasm has a higher genetic diversity level and the genetic relationship is not entirely consistent with geographical distribution and ecological environment.