Research on inhibition mechanism of polypeptide extract from scorpion venom combined with Rapamycin on angiogenesis of H22 hepatoma / 中草药

ABSTRACT

Objective: To study the inhibition of polypeptide extract from scorpion venom (PESV) combined with Rapamycin (RAPA) on angiogenesis of H22 hepatoma and its mechanism. Methods: The H22 carcinoma cell suspension was subcutaneously inoculated into 40 Kunming mice. Then tumor-bearing mice were randomly divided into four groups, i.e., control group, PESV group (20 mg/kg), RAPA group (2.5 mg/kg), and PESV + RAPA group, 10 mice in each group. The intervention was lasted 14 d. The tumor volume was measured once every other day, the tumor volume growth curve was drawn, and the tumor inhibitory rate was calculated. The protein expression levels of mammal target of RAPA (mTOR), hypoxia-inducible factor-1α (HIF-1α), and vascular endothelial growth factor-A (VEGF-A) were detected by immunohistochemical assay. Microvessel was signed by factor VIII and then detected by immunohistochemical assay. Results: The growth speed of H22 hepatoma transplantation tumor was obviously inhibited in the PESV group, RAPA group, and PESV + RAPA group compared with control group after 14 d intervention (P 22 hepatoma, the mechanism maybe related to the inhibition on the protein expression of mTOR, HIF-1α, and VEGF-A.