Study on specific chromatograms of Crocus sativus and authenticity identification / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1378-1380, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-854432
ABSTRACT
Objective:
To establish the method for the specific chromatograms analysis of Crocus sativus, so as to distinguish the active constituents between saffron and gardenia.Methods:
HPLC with ZorBax XDB-C18 column was used, the mobile phase was a linear gradient of methanol containing 0.5% acetic acid and water containing 0.5% acetic acid in 45 min, the detection wavelength was set at 254 nm and the flow-rate was 1.0 mL/min.Results:
Multi batches of samples were analyzed to establish the specific chromatograms. Eight marked peaks were separated. The methodological evaluation showed that the method had a good repeatability. The active constituents between saffron and gardenia could be significantly distinguished by this method.Conclusion:
The method is simple, rapid, and accurate with good reproducibility and can be used for the quality control and identification of C. sativus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2015
Type:
Article
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