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Study on specific chromatograms of Crocus sativus and authenticity identification / 中草药
Chinese Traditional and Herbal Drugs ; (24): 1378-1380, 2015.
Article in Chinese | WPRIM | ID: wpr-854432
ABSTRACT

Objective:

To establish the method for the specific chromatograms analysis of Crocus sativus, so as to distinguish the active constituents between saffron and gardenia.

Methods:

HPLC with ZorBax XDB-C18 column was used, the mobile phase was a linear gradient of methanol containing 0.5% acetic acid and water containing 0.5% acetic acid in 45 min, the detection wavelength was set at 254 nm and the flow-rate was 1.0 mL/min.

Results:

Multi batches of samples were analyzed to establish the specific chromatograms. Eight marked peaks were separated. The methodological evaluation showed that the method had a good repeatability. The active constituents between saffron and gardenia could be significantly distinguished by this method.

Conclusion:

The method is simple, rapid, and accurate with good reproducibility and can be used for the quality control and identification of C. sativus.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2015 Type: Article