Variation analysis on triterpene compounds in hawthorn from different regions / 中草药

ABSTRACT

Objective: To establish an HPLC method for the content determination of ursolic acid and oleanolic acid in hawthorn (Crataegi Fructus) from different regions, whose differences were compared. Methods: Waters Symmetry C18 column (4.6 mm× 250 mm, 5 μm) was used with the mobile phase of acetonitrile-methanol-0.5% ammonium acetate (691219) at a flow rate of 1 mL/min. The detection wavelength was set at 210 nm and the column temperature was 30°C. To determine the content of hawthorn from 10 different regions, variance analysis and cluster analysis were carried out for the results. Results: The linear ranges of ursolic acid and oleanolic acid were 0.078-0.780 mg/mL(r = 0.9998, n = 6) and 0.0169-0.169 mg/mL (r = 0.9996, n = 6), respectively. The average recoveries of the two components were 102.6% and 100.2%, in accordance with the determination requirement in Chinese Pharmacopoeia 2010. Conclusion: This method is simple, quick, accurate, and has better reproducibility for the determination of triterpene compounds in hawthorn. And hawthorn from Chengde and Jiangsu provinces is better. It could be used as the origin of hawthorn in the prescription of Xin mai Capsule.