Preparation of triterpenoid ilexoside O from Ilex pubescens and study on its antithrombotic activity / 中草药

ABSTRACT

Objective: To prepare triterpenoid ilexoside O from the roots of Ilex pubescens and to investigate its antithrombotic activity. Methods: Ilexoside O was separated and purified by column chromatography and recrystallization, and was identified by 1H-NMR and 13C-NMR analyses. Afterig administration, the pharmacological effects of ilexoside O were evaluated by clotting time (CT), bleeding time (BT), and the amount of the abdominal aortic blood clots induced by FeCl3 in rats, respectively. Results: In comparison with placebo group, ilexoside O showed the obvious effects on BT (P<0.01). The low-dose group (25 mg/kg) of ilexoside O showed no obvious effect on CT (P≤0.05), but the mid-(50 mg/kg) and high-(100 mg/kg) dose groups obviously increased CT of mice (P<0.05); Ilexoside O obviously decreased the amount of the abdominal aortic blood clots in the induction of FeCl3 in rats (P<0.01). Conclusion: Ilexoside O shows obvious anticoagulant effect and could decrease the amount of the abdominal aortic blood clots, and its related mechanism is one of the problems which need to be further studied.