Preparation of danshensu-polycaprolactone nanofiber scaffold by electrospinning / 中草药

ABSTRACT

Objective: To prepare Danshensu loaded polycaprolactone (DSS-PCL) nanofiber scaffold by using electrospin technology. Methods: Using dichloromethane-N, N-dimethyl formamide (CH2Cl2-DMF) 82 as solvent, nanofibers containing danshensu was prepared by electrospinning, fiber morphology was observed by SEM, danshensu in vitro release rule from drug loaded nanofiber scaffold was investigated, and the security of nanofiber was assessed by MTT and hemolysis test. Results: By using CH2Cl2-DMF as solvent, drug and materials could be well dissolved and successfully electrospun into nanofiber, the average fiber diameters were 210 and 190 nm, the in vitro release experiment showed that the drug release after a quick release into the slow release. MTT test indicated that PCL and DSS-PCL nanofiber showed no influence to the cell viability, and hemolysis test showed hemolysis ratio of nanofiber was under 5%. Conclusion: DSS-PCL nanofibers is easy to prepare by electrospinning.