Dynamic analysis on endo phylohormone during bolting time of roots in Saponikovia cata / 中草药

ABSTRACT

Objective: Through determining the contents of growth hormone (IAA), gibberellic acid (GA), and abscisic acid (ABA) to make the content changes of IAA, GA, and ABA clear before and after bolting time in the roots of Saponikovia cata, so as to provide the theoretical basis for studying the early bolting mechanism in the roots of S. cata. Methods: The contents of IAA, GA, and ABA before and after the bolting time were determined by HPLC. The chromatographic conditions were as follows C18 ODS RP column, temperature 35°C, detection wavelength 254 nm, volumn flow 0.7 mL/min, mobile phase methanol-acetonitrile -0.7% acetic acid (401545). Results: The content variation trend of IAA and GA in the roots, stems, and leaves of S. cata was almost the same during the bolting time. The contents of IAA and GA in the roots of S. cata revealed the downgrade tendency sharply after bolting; The contents of IAA and GA in the roots of S. cata before bolting were obviously higher than those after bolting. The content variation trends of ABA in the roots, stems, and leaves of S. cata were the same during the bolting time, but far more different from IAA and GA. Although there was the little fluctuation of ABA before bolting, the contents of ABA after bolting were sharply upgraded and were obviously higher than those before bolting. Conclusion: The phenomenon of early bolting in the roots of S. cata directly relates to the endo phylohormone level, so we may extrapolate that the levels of endo phylohormone could straightly cause the bolting in the roots of S. cata or not.