Effects of different Bupleuri Radix compatibility on contents of seven compounds in Chaige Jieji Decoction / 中草药

ABSTRACT

Objective: Bupleuri Radix is a kind of medicinal material derived from many sources, and Bupleurum chinense and B. scorzonerifolium are described in Chinese Pharmacopoeia. In this study, the chemical constituents in Chaige Jieji Decoction (CJD) composed of B. scorzonerifolium or B. chinense were compared to elucidate the influence of different Bupleuri Radix on other components in CJD. Methods: Firstly, an HPLC method for the simultaneous determination of seven constituents (puerarin, albiflorin, paeoniflorin, liquiritin, baicalin, wogonoside, and baicalein) in CJD was developed. Then CJD composed of B. scorzonerifolium or B. chinense was compared by the established method. Results: Good linearity for the seven compounds was obtained, and the average recoveries ranged from 94.1% to 106.6%. The RSD values of all the precision and repeatability of the compounds were less than 2%, and the test sample was stable within 12 h. No significant differences were observed between the two different CJD. Conclusion: The developed HPLC method is simple and repeatable, which is helpful for the quality control of CJD. The effects of different Bupleuri Radix compatibility on other ingredients in CJD and pharmacological activity of CJD deserve further study.