IL-10 Plasmid DNA Delivery in NOD Mice for the Prevention of Autoimmune Pancreatic Beta Cell Destruction / 대한내분비학회지
Journal of Korean Society of Endocrinology
;
: 262-271, 2000.
Article
in Korean
| WPRIM
| ID: wpr-85453
ABSTRACT
BACKGROUND:
Recently, we have reported that biodegradable poly [-(4-aminobutyl)-L-glycolic acid] (PAGA) can condense and protect plasmid DNA from DNase I. In this study, we investigated whether the systemic administration of pCAGGS mouse IL-10 (mIL-10) expression plasmid complexed with PAGA can reduce the development of insulitis in non-obese diabetic (NOD) mice.METHODS:
PAGA/mIL-10 plasmid complexes were stable for more than 60 minutes, but the naked DNA was destroyed within 10 minutes by DNase I. The PAGA/DNA complexes were injected into the tail vein of 3 week-old NOD mice.RESULTS:
Serum mIL-10 level peaked at 5 days after injection, could be detected for more than 7 weeks. The prevalence of severe insulitis at 12 week-old NOD mice was markedly reduced by the intravenous injection of PAGA/DNA complex (15.7%) compared to that of naked DNA injection (34.5%) and non-treated controls (90.9%). In conclusion, systemic administration of pCAGGS mIL-10 plasmid/PAGA complexes can reduce the severity of insulitis in NOD mice.CONCLUSION:
The study presents the PAGA/DNA complex has the potential for the application of the prevention of autoimmune diabetes mellitus.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Veins
/
DNA
/
Genetic Therapy
/
Prevalence
/
Mice, Inbred NOD
/
Interleukin-10
/
Deoxyribonuclease I
/
Diabetes Mellitus, Type 1
/
Insulin-Secreting Cells
Type of study:
Prevalence study
Limits:
Animals
Language:
Korean
Journal:
Journal of Korean Society of Endocrinology
Year:
2000
Type:
Article
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