Cloning and expression analysis of 1-deoxy-D-xylulose-5-phosphate synthase 1 gene in Houttuynia cordata / 中草药

ABSTRACT

Objective: To clone the 1-deoxy-D-xylulose-5-phosphate synthase 1 (DXS1) gene from Houttuynia cordata and to analyze the expression difference. Methods: The cloning primers were designed based on the transcriptome dataset of H. cordata, one unique sequence encoding DXS1 was discovered. The sequence of DXS1 was cloned from H. cordata by RT-PCR. The physicochemical properties, secondary structure, and three-dimensional structure of the DXS1 protein were forecasted and analyzed, and its structure and function were predicted. And the different expression levels of DXS1 gene in rhizome, stems, leaves, and flowers were analyzed by fluorescent quantitative PCR. Results: The cDNA (named as DXS1) contains a 2 172 bp open reading frame and encodes a predicted protein of 723 amino acids. No transmembrane region and signal peptide were present in DXS1. The conserved domain of DXS was present in DXS1. Relative real-time PCR analysis indicated that DXS1 showed the highest transcript abundance in the flowers, moderate level in the leaves, lower level in the rhizomes, and the lowest level in the stems. Conclusion: This study cloned the DXS1 gene from H. cordata for the first time. The results will lay a foundation for exploring the mechanism of terpenoid biosynthesis in H. cordata plants.