Determination for contents of linarin and chlorogenic acid in different parts of Chrysanthemum indicum by HPLC-DAD / 中草药

ABSTRACT

Objective: To analyze the contents of linarin and chlorogenic acid in different parts such as flowers, leaves, young stems, and old stems of Chrysanthemum indicum. To provide the theoretical basis and analysis method for the quality evaluation of different parts of C. indicum. Methods: Thermo C18 column (250 mm × 4.6 mm, 5 μm) was used with a mobile phase of acetonitrile-0.05% phosphoric acid solution in the gradient elution by HPLC-DAD. The flow rate was 1 mL/min, the column temperature was set at 30°C, and the detective wavelength was 327 nm. Results: The linear response of linarin ranged from 0.15 to 3.00 μg (r=0.9990), and that for chlorogenic acid was from 0.01 to 0.20 μg (r=0.9995), the average recovery was 98.17% and 97.99%, respectively. The content of linarin from the leaves was higher, the content of chlorogenic acid from the flowers was higher, and the content of each component in the stems was the lowest. Conclusion: The method is convenient, and the result is accurate and can be used for the quality control of different parts of C. indicum. The results of content determination indicate that there are linarin and chlorogenic acid in all different parts of C. indicum, but the mass fractions were obviously different.