Chemical constituents from rhizoma of Anemone altaica / 中草药

ABSTRACT

Objective: To study the chemical constituents in the rhizoma of Anemone altaica. Methods: The isolation and purification of the compounds were performed by Diaion HP-20 macroporous resin, Sephadex LH-20, and silica gel column chromatography, and their structures were determined by comparing their physicochemical characters and spectral data with literatures. Results: Fourteen compounds were obtained, and their structures were identified, including six phenolic compounds ferulic acid (1), mono-feruloyl-tartaric acid (2), chlorogenic acid (3), glucosyringic acid (4), feruloyl-6'-O-α-D-glucopyranoside (5), and feruloyl-6'-O-β-D-glucopyranoside (6); two lignans (+)-isolariciresino-1-9-O-β-D-glucopyranoside (7) and (+)-pinoresinol-4-O-β-D-glucopyranoside (8); one courmarin esculetin (9); and five nitrogen-containing compounds 2, 3, 4, 9-tetrahydro-1H-pyridine pyrido [3, 4-b] indole-3-carboxylic acid (10), phenylalanine (11), adenine (12), thymidine (13), and adenosine (14). Conclusion: Compounds 2-13 are isolated from the plants of the genus Anemone L. for the first time.