Chemical constituents from stems and leaves of Humulus scandens / 中草药

ABSTRACT

Objective: To study the chemical constituents from the roots and stems of Humulus scandens. Methods: The chromatographies on silica gel column, reverse phase column, and semi-preparative HPLC were used to isolate and purify the constituents and the structures of the compounds were elucidated on the basis of physicochemical properties and spectral data. Results: Ten compounds were obtained from the ethyl acetate fraction of methanol extract in the roots and stems of H. scandens and identitied as N-p-coumaroyltyramine (1), N-trans-feruloyltyramine (2), cosmosiin (3), 3, 3'-dimethoxy-4, 8'-oxyneoligna-9, 4', 7', 9'-tetraol (4), 3-hydroxy-5, 6-epoxy-7-megastigmen-9-one (5), 4, 5-dihydroblumenol (6), scopoletin (7), citruusin C (8), 2-phenylethyl-O-β-D-glucopyranoside (9), and 3-oxo-α-ionol-β-D-glucopyranoside (10). Conclusion: Compounds 2, 4-6, and 8-10 are all obtained from H. scandens for the first time, and compounds 2, 4-6, 9, and 10 are firstly isolated from the plants of this genus. Compounds 2, 4, 7, and 9 show remarkable scavenging activity toward DPPH radical with IC50 values of 0.01, 3.36, 10.55, and 18.15 μg/mL, respectively. The scientific evidence is provided for the development of anti-oxidant and anti-aging aspects of the drugs.