Separation and purification of total poly phenol from Dryopteris crassirhizoma with anion exchange resin / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1265-1269, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-854586
ABSTRACT
Objective:
To optimize the separation and purification of the total poly phenol from Dryopteris crassirhizoma with anion- exchange resin.Methods:
The rates of elution and absorption, contents of dryocrassin ABBA, and total ploy phenol were used as makers to optimize the purification conditions of total ploy phenol.Results:
The extracting solution of D. crassirhizoma passed through 201×7 hydrogen-oxygen the anion-exchange resin column (column diameter-column height, 17) at the rate of 6 BV/h in reverse direction, then the resin column was flushed with water at the rate of 6BV/h to pH value 6-7 in forward direction. At last, the column was eluted at the rate of 6 BV/h to obtain ploy phenol with 9BV 3% salt water in 60% alcohol. The elution ratio of dryocrassin ABBA is 90.1%, and the total ploy phenol is 91.1%. The content of dryocrassin ABBA is 29.4% and the content of the total ploy phenol is 49.2%.Conclusion:
The separation and purification of the total ploy phenol from D. crassirhizoma with 201 × 7 hydrogen-oxygen anion-exchange resin can achieve the satisfactory results which have wide application prospects.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2014
Type:
Article
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