Genetic diversity and quality analysis of cultivated Pesudostellaria heterophylla / 中草药

ABSTRACT

Objective: To study the genetic diversity and medicinal quality of Pesudostellaria heterophylla and to provide a reference for rational utilization on germplasm resources and fine variety breeding of P. heterophylla. Methods: The genetic diversity of the 12 cultivated provenances of P. heterophylla were analyzed by ISSR molecular markers, and Heterophyllin B (HB) in the root of P. heterophylla was analyzed by HPLC. Results: A total of 82 bands were produced by 10 primers, among which 73 bands were polymorphic bands, and the percentage of polymorphic bands (PPL) was 89.02%. The average value of Nei's genetic diversity index (H) was 0.2579, Shannon's information index (I) was 0.3884, genetic differentiation coefficient (Gst) was 0.2741, and the gene flow (Nm) among provenances was 1.3238. Cluster analysis based on genetic identity indicated that 12 provenances could be divided into three groups. There were great differences of HB content among and within provenances. Heterophyllin B content (0.0494%) in provenance 4 was significantly higher than those in the others, and their coefficient of variation (9.51%) was less in this provenance. Conclusion: The high genetic diversity could be attributed to the provenances exchange in different production areas of P. heterophylla and its biological characteristics. Comprehensive consideration of genetic diversity and HB are made, and the provenances 3 and 4 of P. heterophylla have better quality, which are suitable for germplasm conservation and variety breeding.