Simultaneous determination of rutin, hyperin, and isoquercitrin in leaves of Apocynum venetum and Poacynum hendersonii located in Xinjiang by HPLC / 中草药

ABSTRACT

Objective: To establish an HPLC method for the simultaneous determination of the main flavonoids in the leaves of Apocynum venetum and Poacynum hendersonii, and to reveal the differences of flavonoids in Apocyni Veneti Herba located between Xinjiang and other areas in China. Methods: The leaves of the two kinds of Apocyni Veneti Herba were extracted with 60% ethanol by reflux extraction. The separation was carried out on an Agilent TC-C18 column (150 mm × 4.6 mm, 5 μm) eluted with the mobile phases of acetonitrile-0.1% phosphoric acid (1684). The column temperature was 40°C, and the flow rate was 1.0 mL/min, the detection wavelength was 360 nm, and the column temperature was 40°C. Results: The linear ranges of rutin, hyperin, and isoquercitrin were 4.96-49.60 (r = 0.9992), 0.96-9.60 (r = 0.9990) and 10.24-102.40 μg/mL (r = 0.9970), respectively; and the average recoveries (n = 5) were 98.2% (RSD = 1.5%), 92.3% (RSD = 3.2%), and 100.1% (RSD = 2.7%), respectively. Conclusion: This method is simple, rapid, accurate, and reproducible, and the results show that the flavonoids in Apocyni Veneti Herba located in Xinjiang are remarkably different from those in the other areas in China. In the leaves of A. venetum located in Xinjiang, the lutin content is high, about 0.55%, close to the isoquercetin content, but the hypercetin content is very low, only about 0.07%, and in the leaves of P. hendersonii, the contents of both lutin and hypercetin are very small. Such study will offer the credible method for the quality control of A. venetum and P. hendersonii.