Preparing technology of asiaticosides with high-purity / 中草药

ABSTRACT

Objective: To study the preparing technology of asiaticosides with high purity. Methods: Macroreticular resin column chromatography and recrystallization were used to optimize the preparing technology of asiaticosides. HPLC method was established for the contcent determination of asiaticosides. Results: Centella asiatica was subjected to 75% ethanol distilment, water and acid precipitation, HPD100 macroreticular resin separation, and recrystallization in acetic ester. Then asiaticosides with high purity over 90% were obtained. Conclusion: The process is stable and feasible, and it could be used for the preparation of high-purity asiaticosides.