Gene screening of Buyang Huanwu Decoction used for delaying denervated tibial muscle atrophy of rats / 中草药

ABSTRACT

Objective: To screen the target gene of Buyang Huanwu Decoction (BHD) used for delaying denervated tibial muscle atrophy of rats by genechip technique and to verify the differentially expressed gene PI3K. Methods: After 20 Sprague-Dawley (SD) rats were subjected to common peroneal nerve crush model of 5 mm injury, they were randomly divided into BHD and model groups, for daily ig administration of drugs after operation. The drug administration lasted for 18 d, the genechip analysis was performed to screen the differentially expressed gene. Then, RT-PCR and Western-blotting were used to detect the differential expression of PI3K and protein. Results: Compared with the model group, there was a significant difference of gene expression profile in BHD group. Among them, the expression of 14 genes had up-regulation and 10 genes had down-regulation. In the differential expression genes, Angptl4 and Pik3c2g had the more obvious change (up-regulation for 5-fold). Simultaneously, the validation tests showed Compared with the model group, the PI3K mRNA and protein expression in the BHD mid- and high-dose (crude drug 12.96 and 25.92 g/kg) groups increased significantly (P < 0.05, 0.01). Conclusion: The preventive and therapeutic effects of BHD on denervated tibial muscle atrophy of rats are relevant with the regulation of many related genes. The mechanism may be as follows promoting energy synthesis and blood neogenesis, neuroprotection, anti-apoptosis, and inhibition of collage synthesis; BHD could delay the denervated tibial muscle atrophy of rats by increasing the expression of PI3K and protein; The activation of PI3K/Akt signal transduction pathway probably play an important role in delaying the denervated muscle atropy by BHD.