Generation of Multilayered 3D Structures of HepG2 Cells Using a Bio-printing Technique
Gut and Liver
;
: 121-128, 2017.
Article
in English
| WPRIM
| ID: wpr-85468
ABSTRACT
BACKGROUND/AIMS:
Chronic liver disease is a major widespread cause of death, and whole liver transplantation is the only definitive treatment for patients with end-stage liver diseases. However, many problems, including donor shortage, surgical complications and cost, hinder their usage. Recently, tissue-engineering technology provided a potential breakthrough for solving these problems. Three-dimensional (3D) printing technology has been used to mimic tissues and organs suitable for transplantation, but applications for the liver have been rare.METHODS:
A 3D bioprinting system was used to construct 3D printed hepatic structures using alginate. HepG2 cells were cultured on these 3D structures for 3 weeks and examined by fluorescence microscopy, histology and immunohistochemistry. The expression of liver-specific markers was quantified on days 1, 7, 14, and 21.RESULTS:
The cells grew well on the alginate scaffold, and liver-specific gene expression increased. The cells grew more extensively in 3D culture than two-dimensional culture and exhibited better structural aspects of the liver, indicating that the 3D bioprinting method recapitulates the liver architecture.CONCLUSIONS:
The 3D bioprinting of hepatic structures appears feasible. This technology may become a major tool and provide a bridge between basic science and the clinical challenges for regenerative medicine of the liver.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Tissue Donors
/
Immunohistochemistry
/
Gene Expression
/
Cause of Death
/
Liver Transplantation
/
Regenerative Medicine
/
Hep G2 Cells
/
Bioprinting
/
Printing, Three-Dimensional
/
Liver
Limits:
Humans
Language:
English
Journal:
Gut and Liver
Year:
2017
Type:
Article
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