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Optimization of regeneration system and resistance screening of Rhodiola crenulata / 中草药
Chinese Traditional and Herbal Drugs ; (24): 558-562, 2014.
Article in Chinese | WPRIM | ID: wpr-854681
ABSTRACT

Objective:

To optimize the regeneration system of Rhodiola crenulata, to establish the optimal conditions for resistance screening, and to lay the foundation for the establishment of the efficient genetic transformation system of R. crenulata.

Methods:

The leaves of Rhodiola crenulata were used as explants. The influences of different ratios of 6-BA, NAA, and IAA in the medium on callus induction and growth conditions were observed, and the implant resistance of Kanamycin (Kan) and hygromycin (Hyg) was screened by gradient method.

Results:

MS + 3.0 mg/L 6-BA + 1.0 mg/L NAA + 700 mg/L L-Pro was the optimal medium for the differentiation of leaf adventitious bud and the differentiation ratio of adventitious bud reached 92%; MS + 700 mg/L L-Pro was the medium for adventitious roots; The best selection of genetic transformation system for R. crenulata was 200 mg/L Kan and 10 mg/L Hyg; Adding 10 mg/L Vc could effectively inhibit the phenolic substances secretion.

Conclusion:

The regeneration system of R. crenulata, is optimized, and the pressure of Kan and Hyg for genetic transformation system of R. crenulata is screened.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Screening study Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2014 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Screening study Language: Chinese Journal: Chinese Traditional and Herbal Drugs Year: 2014 Type: Article