Bud stem tissue culture of Tulipa edulis / 中草药

ABSTRACT

Objective: To establish the tissue culture system of callus induction and cluster shoot proliferation with bud stems of Tulipa edulis. Methods: Bud stems were isolated from cooled T. edulis bulbs as explants. The calli were inducted on MS media with different concentration of 6-BA and NAA, and the cultural conditions of shoot differentiation and multiplication were optimized. Results: The optimal medium for callus induction was MS + 6-BA 0.5 mg/L + NAA 2.0 mg/L with a callus inducation rate of 78.54%. After subcultured in original medium, the callus was turned into differentiation medium. The optimal medium for callus differentiation was MS + 6-BA 2.0 mg/L + NAA 0.2 mg/L with a shoot differentiation rate of 66.21%. The optimal medium for the shoot multiplication was MS + 6-BA 0.5 mg/L + NAA 0.2 mg/L, and the proliferation coefficient was 2.48. Conclusion: The media for callus induction, adventitious bud differentiation, and cluster shoot proliferation are optimized. The optimal medium for the culture of T. edulis bud stems is preliminarily established.