Bud stem tissue culture of Tulipa edulis / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 563-568, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-854682
ABSTRACT
Objective:
To establish the tissue culture system of callus induction and cluster shoot proliferation with bud stems of Tulipa edulis.Methods:
Bud stems were isolated from cooled T. edulis bulbs as explants. The calli were inducted on MS media with different concentration of 6-BA and NAA, and the cultural conditions of shoot differentiation and multiplication were optimized.Results:
The optimal medium for callus induction was MS + 6-BA 0.5 mg/L + NAA 2.0 mg/L with a callus inducation rate of 78.54%. After subcultured in original medium, the callus was turned into differentiation medium. The optimal medium for callus differentiation was MS + 6-BA 2.0 mg/L + NAA 0.2 mg/L with a shoot differentiation rate of 66.21%. The optimal medium for the shoot multiplication was MS + 6-BA 0.5 mg/L + NAA 0.2 mg/L, and the proliferation coefficient was 2.48.Conclusion:
The media for callus induction, adventitious bud differentiation, and cluster shoot proliferation are optimized. The optimal medium for the culture of T. edulis bud stems is preliminarily established.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Traditional and Herbal Drugs
Year:
2014
Type:
Article
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