Preparation of lyophilized powder of oral Pueraria flavonoids nanosuspension and determination of dissolution rates of its four effective components / 中草药

ABSTRACT

Objective: To prepare the lyophilized powder of Pueraria flavonoids nanosuspension (PF-NS) and to determine the dissolution rates of its four effective components (3'-hydroxypuerarin, puerarin, daidzin, and daidzein). Methods: PF-NS was prepared by the high pressure homogenization (HPH) technology. The lyophilized formula contained mannitol as lyoprotectant. The dissolution rates of the four effective components from lyophilized powder of PF-NS as well as the physical mixture were determined, with artificial gastric juice (pH 1.2) as dissolvent. Results: The optimal lyophilized powder of PF-NS was loosed with the particle size of (479.7 ± 14.7) nm, polydisperse index of 0.524 ± 0.220, and Zeta potential of (29.68 ± 3.97) mV, respectively. The in vitro accumulated dissolution rate of lyophilized powder of PF-NS was higher than that of the physical mixture. Conclusion: The method employed to prepare the lyophilized powder in PF-NS is simple and feasible. The lyophilized powder of PF-NS could improve the in vitro dissolution rate notablely. It might be a novel vehicle potentially for nano-drug delivery system of PF.