Comparison on hemostasis of Platycladi Cacumen before and after processing on blood heat and hemorrhage syndrome rat model / 中草药

ABSTRACT

Objective: To discuss the hemostasis of Platycladi Cacumen (PC) before and after processing on the blood heat and hemorrhage syndrome rat model induced by dry yeast. Methods: The SD rats were divided into five groups. Yunnan Baiyao was taken as the positive control drug. The rats in the control and model groups were fed with CMC-Na for 7 d, and the rats in other groups were fed with corresponding drugs simultaneously. On day 7, the blood heat and hemorrhage syndrome rat model was established. Indexes including the whole blood viscosity, plasma viscosity, thrombin time (TT), activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen content (FIB), red blood cell (RBC), hemoglobin (HGb), hematocrit (HCT), blood platelet count (PLT), thrombocytocrit (PCT), mean platelet volume (MPV), and platelet distribution width (PDW) were detected, and the histopathological examinations of lungs among each group were compared. Results: Compared with the control group, the RBC, HGb, and HCT of rats in the model group increased significantly (P < 0.01), with significant increase in high, middle and low whole blood viscosities and plasma viscosity (P < 0.05, 0.01) of rats in the model group; TT and APTT were notably prolonged, while PT was notably shortened, with significant increase in FIB content (P < 0.01); Additionally, PLT, PCT, MPV, and PDW remarkably increased (P < 0.05, 0.01). After ig administration of PC or Platycladi Cacumen Carbonisatum (PCC), the low whole blood viscosities and plasma viscosity were remarkably decreased (P < 0.05, 0.01), and RBC, HGb, and HCT were remarkably decreased (P < 0.05, 0.01); TT and APTT of PC group were remarkably shortened (P < 0.05, 0.01), with remarkable decrease in FIB content (P < 0.05); TT and APTT of PCC group were significantly shortened (P < 0.01), with significant decrease in FIB content (P < 0.01); Meanwhile, PLT, PCT, MPV, and PDW of PCC group remarkably decreased (P < 0.05); The injury of lungs was also improved in both groups. Conclusion: Both PC and PCC show the effect of hemostasis by ameliorating hemorheological parameters and platelet parameters to different degrees, improving coagulation function mainly by acting on the endogenous coagulation. Simultaneously, they could ameliorate the damaged lungs, but the therapeutical effect is stronger after being carbonized.